FASL ELISA Kit

Catalog No. ABIN6719985

Product Details FASL ELISA Kit

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Apoptosis (APO 1) antigen ligand 1, Apoptosis antigen ligand, APT1LG1, APTL, CD178, CD95 L, CD95 ligand, CD95L, CD95L protein, Fas antigen ligand, FASL, FASLG, TNF superfamily member 6, TNFSF6, Tumor necrosis factor (ligand) superfamily member 6, Tumor necrosis factor ligand superfamily member 6

Background: Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

Gene Name: FASLG

Production: Natural and recombinant human FASL. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: CHO, Immunogen sequence: P134-L281

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human FASL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human FASL standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: FASL (FASL Products)

Synonyms: ALPS1B ELISA Kit, APT1LG1 ELISA Kit, APTL ELISA Kit, CD178 ELISA Kit, CD95-L ELISA Kit, CD95L ELISA Kit, FASL ELISA Kit, TNFSF6 ELISA Kit, fasl ELISA Kit, Fas-L ELISA Kit, Faslg ELISA Kit, Tnfsf6 ELISA Kit, gld ELISA Kit, Apt1Lg1 ELISA Kit, Fasl ELISA Kit, FASLG ELISA Kit, zgc:162027 ELISA Kit, Fas ligand ELISA Kit, Fas ligand L homeolog ELISA Kit, Fas ligand (TNF superfamily, member 6) ELISA Kit, FASLG ELISA Kit, faslg.L ELISA Kit, Fasl ELISA Kit, Faslg ELISA Kit, faslg ELISA Kit

Gene ID: 14103

NCBI Accession: NP_001192172

UniProt: Q99PH8

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity