CCL16 ELISA Kit

Catalog No. ABIN6719980

Product Details CCL16 ELISA Kit

Target: CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8 pg/mL - 500 pg/mL

Minimum Detection Limit: 7.8 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: C-C motif chemokine 16, CCL16, CCL16_HUMAN, Chemokine CC-4, Chemokine LEC, CKb12, HCC-4, HCC4, IL-10-inducible chemokine, IL10 inducible chemokine, ILINCK, LCC-1, LCC1, Liver CC chemokine 1 precursor, Liver expressed chemokine, Liver-expressed chemokine, LMC, Lymphocyte and monocyte chemoattractant, Monotactin 1, MTN-1, Mtn1,NCC4, New CC chemokine 4, SCYA16, SCYL4, Small inducible cytokine A16 precursor, Small inducible cytokine subfamily A (Cys Cys) member 16, Small-inducible cytokine A16

Background: Chemokine(C-C motif) ligand 16(CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine(LEC) and Monotactin-1(MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8.

Gene Name: CCL16

Production: Natural and recombinant human CCL16. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: Q24-Q120

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human CCL16 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human CCL16 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 7.8pg/mL-500pg/mL

Other Performance Data: DOE: 2-8° C - 2/15/18 -20° C - 8/16/18

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CCL16

Target: CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))

Alternative Name: CCL16 - HCC-4 (CCL16 Products)

Synonyms: CKb12 ELISA Kit, HCC-4 ELISA Kit, ILINCK ELISA Kit, LCC-1 ELISA Kit, LEC ELISA Kit, LMC ELISA Kit, Mtn-1 ELISA Kit, NCC-4 ELISA Kit, NCC4 ELISA Kit, SCYA16 ELISA Kit, SCYL4 ELISA Kit, C-C motif chemokine ligand 16 ELISA Kit, CCL16 ELISA Kit, Ccl16 ELISA Kit

Gene ID: 6360

NCBI Accession: NP_004581

UniProt: O15467