PLAU ELISA Kit

Catalog No. ABIN6719975

Product Details PLAU ELISA Kit

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: ATF, BDPLT5, Plasminogen activator urinary, PLAU, QPD, u PA, U-plasminogen activator, uPA, URK, UROK_HUMAN, Urokinase-type plasminogen activator chain B

Background: Plasminogen activator,urokinase(PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease(AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell(VSMC), uPA stimulates migration via the uPA receptor(uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase(PI3-K).

Gene Name: PLAU

Production: Natural and recombinant human Upa. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: M1-L431

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human uPA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human uPA standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for PLAU

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Alternative Name: uPA - PLAU (PLAU Products)

Synonyms: PLAU ELISA Kit, u-PA ELISA Kit, ATF ELISA Kit, BDPLT5 ELISA Kit, QPD ELISA Kit, UPA ELISA Kit, URK ELISA Kit, uPA ELISA Kit, UPAM ELISA Kit, plasminogen activator, urokinase ELISA Kit, urokinase-type plasminogen activator ELISA Kit, PLAU ELISA Kit, CpipJ_CPIJ002131 ELISA Kit, CpipJ_CPIJ006543 ELISA Kit, CpipJ_CPIJ013396 ELISA Kit, CpipJ_CPIJ013623 ELISA Kit, Plau ELISA Kit

Gene ID: 5328

NCBI Accession: NP_001138503

UniProt: P00749

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration