Survivin ELISA Kit
-
- Target See all Survivin (BIRC5) ELISA Kits
- Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))
-
Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 62.5 pg/mL - 4000 pg/mL
- Minimum Detection Limit
- 62.5 pg/mL
- Application
- ELISA
- Supplier Product No.
- koa0369
- Supplier
- Rockland
- Purpose
- Human Survivin Sandwich ELISA Kit for Quantitative Detection
- Brand
- AccuSignal™
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Specificity
-
Production: Natural and recombinant human Survivin. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 2 pg/mL
- Components
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product BIRC5 ELISA Kit
-
-
- Application Notes
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Survivin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Survivin standard solution and each sample be measured in duplicate.
- Comment
-
Standard: Expression system for standard: E.coli, Immunogen sequence: M1-D142
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
-
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
-
- Target See all Survivin (BIRC5) ELISA Kits
- Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))
- Alternative Name
- Survivin (BIRC5 Products)
- Background
-
Synonyms: API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, Baculoviral IAP repeat containing 5, Baculoviral IAP repeat-containing protein 5, BIRC5, BIRC5_HUMAN, EPR 1
Background: Survivin(also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50 % of high-grade non-Hodgkin's lymphomas. Survivin encodes a deduced 142-amino acid protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant human Survivin with the molecular mass of 34 KDa.
- Gene ID
- 332
- NCBI Accession
- NP_001012270
- UniProt
- O15392
- Pathways
- Apoptosis, Cell Division Cycle, Nuclear Hormone Receptor Binding
-