Prolyl Endopeptidase FAP ELISA Kit
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- Target See all Prolyl Endopeptidase FAP (FAP) products
- Prolyl Endopeptidase FAP (FAP)
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 62.5 pg/mL - 4000 pg/mL
- Minimum Detection Limit
- 62.5 pg/mL
- Application
- ELISA
- Purpose
- Sandwich ELISA for Quantitative Detection of Antigen
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Characteristics
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Synonyms: 170 kDa melanoma membrane-bound gelatinase, FAP, Fibroblast activation protein alpha, Integral membrane serine protease, SEPR_HUMAN, Seprase
Background: FAP(Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50 % of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48 % amino acid identity with dipeptidyl peptidase IV and 30 % identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2 % of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.
Gene Name: FAP
Production: Natural and recombinant human Seprase. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: L26-D760
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- Application Notes
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Seprase standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Seprase standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 62.5pg/mL-4000pg/mL
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Target See all Prolyl Endopeptidase FAP (FAP) products
- Prolyl Endopeptidase FAP (FAP)
- Alternative Name
- Seprase - FAP (FAP Products)
- Synonyms
- DPPIV ELISA Kit, FAPA ELISA Kit, fibroblast activation protein alpha ELISA Kit, FAP ELISA Kit
- Gene ID
- 2191
- NCBI Accession
- NP_001278736
- UniProt
- Q12884
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