CCL7 ELISA Kit

Catalog No. ABIN6719963

Product Details CCL7 ELISA Kit

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: C-C motif chemokine 7, Ccl7, CCL7_HUMAN, Chemokine CC Motif Ligand 7, Chemotactic protein 3, FIC, Intercrine/chemokine MARC, MARC, MCP 3, MCP-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, RANTES, sis homolog, RP23-350G1.4, SCYA6, SCYA7, Small inducible cytokine A7, Small-inducible cytokine A7

Background: Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Gene Name: CCL7

Production: Natural and recombinant rat CCL7. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: Q24-P97

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat CCL7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat CCL7 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: CCL7 - MCP-3 (CCL7 Products)

Synonyms: CCL7 ELISA Kit, FIC ELISA Kit, MARC ELISA Kit, MCP-3 ELISA Kit, MCP3 ELISA Kit, NC28 ELISA Kit, SCYA6 ELISA Kit, SCYA7 ELISA Kit, Scya7 ELISA Kit, fic ELISA Kit, marc ELISA Kit, mcp3 ELISA Kit, C-C motif chemokine ligand 7 ELISA Kit, chemokine (C-C motif) ligand 7 ELISA Kit, CCL7 ELISA Kit, Ccl7 ELISA Kit

Gene ID: 287561

NCBI Accession: NP_001007613

UniProt: Q9QXY8