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CXCL2 ELISA Kit

Rockland koa0260 CXCL2 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Catalog No. ABIN6719957
Supplier Product No.: koa0260
  • Target See all CXCL2 ELISA Kits
    CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))
    Reactivity
    • 16
    • 14
    • 12
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6 pg/mL - 1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0260
    Supplier
    Rockland
    Purpose
    Mouse MIP-2 Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant mouse MIP-2. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 5 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product CXCL2 ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse MIP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse MIP-2 standard solution and each sample be measured in duplicate.
    Comment

    Standard: Expression system for standard: E.coli, Immunogen sequence:  A28-N100

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all CXCL2 ELISA Kits
    CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))
    Alternative Name
    MIP-2 (CXCL2 Products)
    Background

    Synonyms: C-X-C motif chemokine 2, Chemokine (C-X-C motif) ligand 2, CINC-2a, Cxcl2, CXCL2_HUMAN, Gro beta, Gro-beta, GRO-beta(5-73), GRO-beta-T, GRO2, GRO2 oncogene, GROb, Growth-regulated protein beta, Hematopoietic synergistic factor, HSF, Macrophage inflammatory protein 2-alpha, Melanoma growth stimulatory activity beta, MGSA beta, MGSA-b, MIP 2, MIP 2a, MIP2, MIP2-alpha, MIP2A, ,SB-251353, SCYB2

    Background: MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein(MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein(MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water(aquaporins), glycerol and other small molecules in microbial, plant and animal cells. The standard used in this kit is recombinant mouse MIP-2(A28-N100), consisting of 73 amino acids with the molecular mass of 8KDa.

    Gene ID
    20310
    NCBI Accession
    NP_033166
    UniProt
    P10889
    Pathways
    Cellular Response to Molecule of Bacterial Origin
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