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CCL6 ELISA Kit

CCL6 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Catalog No. ABIN6719953
  • Target See all CCL6 ELISA Kits
    CCL6 (Chemokine (C-C Motif) Ligand 6 (CCL6))
    Reactivity
    • 5
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6 pg/mL - 1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    Sandwich ELISA for Quantitative Detection of Antigen
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Characteristics

    Synonyms: C-C motif chemokine 6, c10, CC chemokine C10, Ccl6, CCL6(23-95), CCL6_MOUSE, Chemokine (C-C motif) ligand 6, MRP 1, Protein C10, Scya6, Small-inducible cytokine A6

    Background: Chemokine(C-C motif) ligand 6(CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents.In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin(e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

    Gene Name: Ccl6

    Production: Natural and recombinant mouse CCL6. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: G22-A116

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  • Application Notes

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CCL6 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CCL6 standard solution and each sample is measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 15.6pg/mL-1000pg/mL

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target See all CCL6 ELISA Kits
    CCL6 (Chemokine (C-C Motif) Ligand 6 (CCL6))
    Alternative Name
    CCL6 - C10 (CCL6 Products)
    Synonyms
    MRP-1 ELISA Kit, Scya6 ELISA Kit, c10 ELISA Kit, Abcc1a ELISA Kit, Avcc1a ELISA Kit, Mrp ELISA Kit, Mrp1 ELISA Kit, Mrp-1 ELISA Kit, Scay6 ELISA Kit, chemokine (C-C motif) ligand 6 ELISA Kit, ATP binding cassette subfamily C member 1 ELISA Kit, Ccl6 ELISA Kit, Abcc1 ELISA Kit
    Gene ID
    20305
    NCBI Accession
    NP_033165
    UniProt
    P27784
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