CCL6 ELISA Kit

Catalog No. ABIN6719953

Product Details CCL6 ELISA Kit

Target: CCL6 (Chemokine (C-C Motif) Ligand 6 (CCL6))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: C-C motif chemokine 6, c10, CC chemokine C10, Ccl6, CCL6(23-95), CCL6_MOUSE, Chemokine (C-C motif) ligand 6, MRP 1, Protein C10, Scya6, Small-inducible cytokine A6

Background: Chemokine(C-C motif) ligand 6(CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents.In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin(e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

Gene Name: Ccl6

Production: Natural and recombinant mouse CCL6. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: G22-A116

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CCL6 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CCL6 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CCL6

Target: CCL6 (Chemokine (C-C Motif) Ligand 6 (CCL6))

Alternative Name: CCL6 - C10 (CCL6 Products)

Synonyms: MRP-1 ELISA Kit, Scya6 ELISA Kit, c10 ELISA Kit, Abcc1a ELISA Kit, Avcc1a ELISA Kit, Mrp ELISA Kit, Mrp1 ELISA Kit, Mrp-1 ELISA Kit, Scay6 ELISA Kit, chemokine (C-C motif) ligand 6 ELISA Kit, ATP binding cassette subfamily C member 1 ELISA Kit, Ccl6 ELISA Kit, Abcc1 ELISA Kit

Gene ID: 20305

NCBI Accession: NP_033165

UniProt: P27784