IL-7 ELISA Kit

Catalog No. ABIN6719941

Product Details IL-7 ELISA Kit

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: IL 7, IL7 nirs variant 6, Interleukin 7, Interleukin8

Background: Interleukin 7(IL7) is a protein that is encoded by the IL7 gene in humans. The gene for human interleukin 7(IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.1IL-7 is essential for survival and expansion of pathogenic T helper type 17(T(H)17) cells in experimental autoimmune encephalomyelitis(EAE).2

Gene Name: IL7

Production: Natural and recombinant mouse IL-7. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: E26-I154

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each mouse IL-7 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Sodium EDTA

ELISA Dilution: 15.6pg/mL-1000pg/mL

Other Performance Data: DOE: 2-8° C - 02/15/19 -20° C - 08/15/19

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: IL-7 (IL7 Products)

Synonyms: IL7 ELISA Kit, IL-7 ELISA Kit, A630026I06Rik ELISA Kit, Il-7 ELISA Kit, hlb368 ELISA Kit, interleukin 7 ELISA Kit, IL7 ELISA Kit, Il7 ELISA Kit

Gene ID: 16196

NCBI Accession: NP_032397

UniProt: P10168

Pathways: JAK-STAT Signaling