FSTL1 ELISA Kit

Catalog No. ABIN6719929

Product Details FSTL1 ELISA Kit

Target: FSTL1 (Follistatin-Like 1 (FSTL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: FLJ50214, FLJ52277, follistatin like 1, follistatin related protein, Follistatin-like protein 1, Follistatin-related protein 1, FRP, FSL1, FSTL1, FSTL1_HUMAN, Micro RNA 198, MIR198, miRNA198 included

Background: Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence(GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.

Gene Name: FSTL1

Production: Natural and recombinant human FSTL1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: M1-I308

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human FSTL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human FSTL1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 312pg/mL-20000pg/mL

Other Performance Data: DOE: 2-8° C- 5/2/18 -20° C - 11/2/18

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for FSTL1

Target: FSTL1 (Follistatin-Like 1 (FSTL1))

Alternative Name: FSTL1 (FSTL1 Products)

Synonyms: FRP ELISA Kit, FSL1 ELISA Kit, MIR198 ELISA Kit, AI316791 ELISA Kit, AW107808 ELISA Kit, Fstl ELISA Kit, TSC-36 ELISA Kit, Frp ELISA Kit, fstl1 ELISA Kit, wu:fc75h02 ELISA Kit, zgc:110703 ELISA Kit, FLIK ELISA Kit, FSTL1 ELISA Kit, DKFZp469P101 ELISA Kit, follistatin like 1 ELISA Kit, follistatin-like 1 ELISA Kit, follistatin-like 1a ELISA Kit, Follistatin-related protein 1 ELISA Kit, follistatin-related protein 1 ELISA Kit, follistatin like 1 L homeolog ELISA Kit, FSTL1 ELISA Kit, Fstl1 ELISA Kit, fstl1a ELISA Kit, fstl1 ELISA Kit, LOC101909195 ELISA Kit, fstl1.L ELISA Kit

Gene ID: 11167

NCBI Accession: NP_009016

UniProt: Q12841