CEA ELISA Kit

Catalog No. ABIN6719926

Supplier Product No.: koa0544

Product Details CEA ELISA Kit

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Supplier Product No.: koa0544

Supplier: Rockland

Purpose: Human CEA Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CEA. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CEA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human CEA standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: K35-A685

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CEA

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Alternative Name: CEA (CEA Products)

Background:

Synonyms: Carcinoembryonic antigen , Carcinoembryonic antigen related cell adhesion molecule 5, CD66e, CD66e antigen, CEA, CEACAM 5, CEACAM5, DKFZp781M2392, Meconium antigen 100

Background: Carcinoembryonic antigen(CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60 % carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon. Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

Gene ID: 1048

NCBI Accession: NP_001278413

UniProt: P06731