Cadherin 5 ELISA Kit
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- Target See all Cadherin 5 (CDH5) ELISA Kits
- Cadherin 5 (CDH5)
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 312 pg/mL - 20000 pg/mL
- Minimum Detection Limit
- 312 pg/mL
- Application
- ELISA
- Supplier Product No.
- koa0790
- Supplier
- Rockland
- Purpose
- Human VE-Cadherin - CD144 Sandwich ELISA Kit for Quantitative Detection
- Brand
- AccuSignal™
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Specificity
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Production: Natural and recombinant human VE-Cadherin. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 10 pg/mL
- Components
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product CDH5 ELISA Kit
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- Application Notes
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human VE-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human VE-Cadherin standard solution and each sample be measured in duplicate.
- Comment
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Standard: Expression system for standard: NSO, Immunogen sequence: D48-Q599
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target See all Cadherin 5 (CDH5) ELISA Kits
- Cadherin 5 (CDH5)
- Alternative Name
- VE-Cadherin/CD144 (CDH5 Products)
- Background
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Synonyms: 7B 4, 7B4, 7B4 antigen, CADH5_HUMAN, Cadherin 5, Cadherin 5 type 2, Cadherin 5, type 2 (vascular endothelium), Cadherin 5, type 2, VE cadherin (vascular epithelium), cadherin vascular endothelial, cadherin vascular endothelial 1, Cadherin-5, Cadherin5, CD 144, CD144, CD144 antigen, CDH 5, CDH5, CDH5 protein, Endothelial specific cadherin, FLJ17376, OTTHUMP00000174777, Vascular endothelial cadherin, Vascular epithelium cadherin, VE Cad, VE-cadherin, VEC
Background: Cadherin 5, type 2 or VE-cadherin also known as CD144, is encoded by the gene CDH5. It is a classical cadherin from the cadherin superfamily. This gene is mapped to mouse chromosome 8. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It has found that PECAM1, VE-cadherin and VEGFR2 together are sufficient to confer responsiveness to flow in heterologous cells.
- Gene ID
- 1003
- NCBI Accession
- NP_001786
- UniProt
- P33151
- Pathways
- Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2
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