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CXCL7 ELISA Kit

Rockland koa0426 PPBP Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Catalog No. ABIN6719909
Supplier Product No.: koa0426
  • Target See all CXCL7 (PPBP) ELISA Kits
    CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
    Reactivity
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6 pg/mL - 1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0426
    Supplier
    Rockland
    Purpose
    Human CXCL7 Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human CXCL7. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 2 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product PPBP ELISA Kit
  • Application Notes

    ELISA_Dilution: 15.6pg/mL-1000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CXCL7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each human CXCL7 standard solution and each sample be measured in duplicate.

    Comment

    Standard: Expression system for standard: E.coli, Immunogen sequence: A59-D128

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all CXCL7 (PPBP) ELISA Kits
    CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
    Alternative Name
    CXCL7 (PPBP Products)
    Background

    Synonyms: B TG1, Beta TG, Beta thromboglobulin, C X C motif chemokine 7, Connective tissue activating peptide III, CTAP3, CTAPIII, LA PF4, LDGF, Leukocyte derived growth factor, Low affinity platelet factor IV, Macrophage derived growth factor, MDGF, NAP 2, Neutrophil activating peptide 2, PBP, Platelet basic protein, Ppbp, pro platelet basic protein, Small inducible cytokine B7, Small inducible cytokine subfamily B member 7, TC1, TC2, TCK 1, TGB, TGB1, THBGB, THBGB1, Thrombocidin 1, Thrombocidin 2, Thymus Chemokine 1

    Background: Chemokine(C-X-C motif) ligand 7(CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine(C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein(PBP) and connective tissue-activating peptide III(CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

    Gene ID
    5473
    NCBI Accession
    NP_002695
    UniProt
    P02775
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