LOXL2 ELISA Kit

Catalog No. ABIN6719903

Product Details LOXL2 ELISA Kit

Target: LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Characteristics:

Synonyms: LOR 2, LOR2, LOX L2, LOXL 2, LOXL2, LOXL2_HUMAN, Lysyl oxidase homolog 2, Lysyl oxidase like 2, Lysyl oxidase like protein 2, Lysyl oxidase related 2, Lysyl oxidase related protein 2, Lysyl oxidase related protein WS9 14, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14, WS9 14

Background: Lysyl oxidase homolog 2 is an enzyme that in humans is encoded by the LOXL2 gene. This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. LOXL2 can also crosslink collagen type IV and hence influence the sprouting of new blood vessels.

Gene Name: LOXL2

Production: Natural and recombinant human LOXL2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q26-Q774

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human LOXL2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human LOXL2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for LOXL2

Target: LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Alternative Name: LOXL2 (LOXL2 Products)

Synonyms: LOR2 ELISA Kit, WS9-14 ELISA Kit, CG4402 ELISA Kit, Dmel\\CG4402 ELISA Kit, Dmloxl-2 ELISA Kit, dmlox-2 ELISA Kit, 1110004B06Rik ELISA Kit, 4930526G11Rik ELISA Kit, 9430067E15Rik ELISA Kit, GB13360 ELISA Kit, lox2-like ELISA Kit, LOXL2 ELISA Kit, lor2 ELISA Kit, loxl-2 ELISA Kit, ws9-14 ELISA Kit, im:7136137 ELISA Kit, si:dkeyp-32b1.1 ELISA Kit, wu:fk12g04 ELISA Kit, zgc:158414 ELISA Kit, lysyl oxidase like 2 ELISA Kit, lysyl oxidase-like 2 ELISA Kit, lysyl oxidase homolog 4 ELISA Kit, lysyl oxidase like 2 L homeolog ELISA Kit, lysyl oxidase-like 2b ELISA Kit, LOXL2 ELISA Kit, lox2 ELISA Kit, Loxl2 ELISA Kit, LOC408544 ELISA Kit, loxl2.L ELISA Kit, loxl2 ELISA Kit, loxl2b ELISA Kit

Gene ID: 4017

NCBI Accession: NP_002309

UniProt: Q9Y4K0

Pathways: Chromatin Binding