Kallikrein 12 ELISA Kit

Catalog No. ABIN6719901

Supplier Product No.: koa0670

Product Details Kallikrein 12 ELISA Kit

Target: Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Supplier Product No.: koa0670

Supplier: Rockland

Purpose: Human KLK12 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human KLK12. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK12 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: A18-N248

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Kallikrein 12

Target: Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))

Alternative Name: KLK12 (KLK12 Products)

Background:

Synonyms: DKFZp686H1078, Kallikrein like protein 5, Kallikrein related peptidase 12, Kallikrein12, KLK 12, KLK L5, KLK12, KLKL5, MGC42603

Background: Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.

Gene ID: 43849

NCBI Accession: NP_062544

UniProt: Q9UKR0

Pathways: Complement System