IL1RL1 ELISA Kit

Catalog No. ABIN6719899

Product Details IL1RL1 ELISA Kit

Target: IL1RL1 (Interleukin 1 Receptor-Like 1 (IL1RL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Characteristics:

Synonyms: DER-4,DER4, FIT 1, Growth stimulation expressed, homolog of mouse growth stimulation-expressed, Il1rl1, IL33R, ILRL1_HUMAN, Interleukin 1 receptor like 1, interleukin 1 receptor related protein, Interleukin-1 receptor-like 1, Ly84, Lymphocyte antigen 84, Protein ST2, ST2, ST2L, ST2V, Ste2, Suppressor of tumorigenicity 2, T1, T1 protein

Background: Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I(IL1R1), interleukin 1 receptor, type II(IL1R2) and interleukin 1 receptor-like 2(IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

Gene Name: IL1RL1

Production: Natural and recombinant human IL1RL1 as well as free IL1RL1 and IL-33 complexed IL1RL1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: K19-S328

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IL1RL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL1RL1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for IL1RL1

Target: IL1RL1 (Interleukin 1 Receptor-Like 1 (IL1RL1))

Alternative Name: IL1RL1 - ST2 (IL1RL1 Products)

Synonyms: DER4 ELISA Kit, Fit-1 ELISA Kit, Ly84 ELISA Kit, ST2L ELISA Kit, St2 ELISA Kit, St2-rs1 ELISA Kit, T1 ELISA Kit, T1/ST2 ELISA Kit, FIT-1 ELISA Kit, IL33R ELISA Kit, ST2 ELISA Kit, ST2V ELISA Kit, FIT1 ELISA Kit, interleukin 1 receptor-like 1 ELISA Kit, interleukin 1 receptor like 1 ELISA Kit, Il1rl1 ELISA Kit, IL1RL1 ELISA Kit

Gene ID: 9173

NCBI Accession: NP_001269337

UniProt: Q01638