IGF2R ELISA Kit

Catalog No. ABIN6719898

Supplier Product No.: koa0796

Product Details IGF2R ELISA Kit

Target: IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Supplier Product No.: koa0796

Supplier: Rockland

Purpose: Human IGF2R Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human IGF2R. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IGF2R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IGF2R standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: S1510-F2108

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IGF2R

Target: IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

Alternative Name: IGF2R (IGF2R Products)

Background:

Synonyms: 300 kDa mannose 6 phosphate receptor, Cation independent mannose 6 phosphate receptor, CD222, CD222 antigen, CI Man 6 P receptor, CI-M6PR, CI-MPR, CIMPR, IGF 2 receptor, IGF 2R, IGF II receptor, IGF2 receptor, IGF2R, Insulin like growth factor 2 receptor, Insulin like growth factor II receptor, M6P R, M6P/IGF2 receptor, M6P/IGF2R, M6PR, mannose 6 phosphate receptor, mannose 6 phosphate receptor cation independent, MPR300, MPRI, MPRI_HUMAN

Background: Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele.

Gene ID: 3482

NCBI Accession: NP_000867

UniProt: P11717