CD320 ELISA Kit

Catalog No. ABIN6719888

Supplier Product No.: koa0740

Product Details CD320 ELISA Kit

Target: CD320 (CD320 Molecule (CD320))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Supplier Product No.: koa0740

Supplier: Rockland

Purpose: Human CD320 - 8D6A Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CD320. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD320 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human CD320 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: S36-Y229

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CD320

Target: CD320 (CD320 Molecule (CD320))

Alternative Name: CD320/8D6A (CD320 Products)

Background:

Synonyms: 8D6, 8D6 antigen, 8D6A, CD320, CD320 antigen, CD320_HUMAN, FDC-signaling molecule 8D6, FDC-SM-8D6, TCblR, Transcobalamin receptor

Background: CD320(cluster of differentiation 320), also known as 8D6A or TCBLR, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin(vitamin B12), and the expression of CD320 enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the CD320 signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. CD320 is also a specific receptor for uptake of TCN2-bound cobalamin.

Gene ID: 51293

NCBI Accession: NP_001159367

UniProt: Q9NPF0