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CD320 ELISA Kit

CD320 Reactivity: Human Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
Catalog No. ABIN6719888
  • Target See all CD320 ELISA Kits
    CD320 (CD320 Molecule (CD320))
    Reactivity
    • 5
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156 pg/mL - 10000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich ELISA for Quantitative Detection of Antigen
    Sample Type
    Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Characteristics

    Synonyms: 8D6, 8D6 antigen, 8D6A, CD320, CD320 antigen, CD320_HUMAN, FDC-signaling molecule 8D6, FDC-SM-8D6, TCblR, Transcobalamin receptor

    Background: CD320(cluster of differentiation 320), also known as 8D6A or TCBLR, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin(vitamin B12), and the expression of CD320 enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the CD320 signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. CD320 is also a specific receptor for uptake of TCN2-bound cobalamin.

    Gene Name: CD320

    Production: Natural and recombinant human CD320. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: S36-Y229

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  • Application Notes

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD320 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human CD320 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 156pg/mL-10,000pg/mL

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target See all CD320 ELISA Kits
    CD320 (CD320 Molecule (CD320))
    Alternative Name
    CD320 - 8D6A (CD320 Products)
    Synonyms
    8D6 ELISA Kit, 8D6A ELISA Kit, TCBLR ELISA Kit, 425O18-1 ELISA Kit, D17Ertd716e ELISA Kit, NG29 ELISA Kit, VLDL ELISA Kit, RGD1305860 ELISA Kit, TCblR ELISA Kit, CD320 molecule ELISA Kit, CD320 antigen ELISA Kit, CD320 ELISA Kit, Cd320 ELISA Kit
    Gene ID
    51293
    NCBI Accession
    NP_001159367
    UniProt
    Q9NPF0
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