Cathepsin E ELISA Kit

Catalog No. ABIN6719886

Product Details Cathepsin E ELISA Kit

Target: Cathepsin E (CTSE)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Characteristics:

Synonyms: CATE, CATE_HUMAN, Cathepsin E, Cathepsin E form II, CTSE, EC 3.4.23.34, Erythrocyte membrane aspartic proteinase, Slow moving proteinase

Background: Cathepsin E is a protein that in humans is encoded by the CTSE gene. It is mapped to 1q32.1. The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. Cathepsin E is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen, and it also has an important role in immune responses.

Gene Name: CTSE

Production: Natural and recombinant human Cathepsin E. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q18-P396

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Cathepsin E standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Cathepsin E standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Cathepsin E

Target: Cathepsin E (CTSE)

Alternative Name: Cathepsin E (CTSE Products)

Synonyms: CATE ELISA Kit, A430072O03Rik ELISA Kit, C920004C08Rik ELISA Kit, CE ELISA Kit, CatE ELISA Kit, CEA ELISA Kit, CEB ELISA Kit, Ctsea ELISA Kit, CTSE ELISA Kit, ce1 ELISA Kit, ce2 ELISA Kit, cate ELISA Kit, CE1 ELISA Kit, ce1-A ELISA Kit, ctse ELISA Kit, CE2 ELISA Kit, ce2-A ELISA Kit, cathepsin E ELISA Kit, cathepsin E L homeolog ELISA Kit, cathepsin E S homeolog ELISA Kit, CTSE ELISA Kit, Ctse ELISA Kit, TP03_0056 ELISA Kit, Smp_184930 ELISA Kit, ctse ELISA Kit, ctse.L ELISA Kit, ctse.S ELISA Kit

Gene ID: 1510

NCBI Accession: NP_001304260

UniProt: P14091