MERTK ELISA Kit
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- Target See all MERTK ELISA Kits
- MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 78 pg/mL - 5000 pg/mL
- Minimum Detection Limit
- 78 pg/mL
- Application
- ELISA
- Supplier Product No.
- koa0482
- Supplier
- Rockland
- Purpose
- Mouse MER - MERTK Sandwich ELISA Kit for Quantitative Detection
- Brand
- AccuSignal™
- Sample Type
- Cell Culture Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
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Production: Natural and recombinant mouse MER. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 10 pg/mL
- Components
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product MERTK ELISA Kit
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- Application Notes
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse MER standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates, tissue homogenates or serum to each empty well. We recommend that each mouse MER standard solution and each sample is measured in duplicate.
- Comment
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Standard: Expression system for standard: sf21, Immunogen sequence: E23-F498
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target See all MERTK ELISA Kits
- MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))
- Alternative Name
- MER/MERTK (MERTK Products)
- Background
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Synonyms: C mer, c mer proto oncogene tyrosine kinase, MER receptor tyrosine kinase, Mertk, MERTK_HUMAN, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK, RP38, STK kinase, Tyrosine-protein kinase Mer
Background: Proto-oncogene tyrosine-protein kinase MER, also called MERTK is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type(immunoglobulin-like) domains, and one tyrosine kinase domain. This gene is mapped to 2q13. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium(RPE) as a regulator of rod outer segments fragments phagocytosis. This gene also plays an important role in inhibition of Toll-like receptors(TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
- Gene ID
- 17289
- NCBI Accession
- NP_032613
- UniProt
- Q60805
- Pathways
- RTK Signaling
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