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P-Cadherin ELISA Kit

Rockland koa0382 CDH3 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
Rockland
Catalog No. ABIN6719852
Supplier Product No.: koa0382
  • Target See all P-Cadherin (CDH3) ELISA Kits
    P-Cadherin (CDH3)
    Reactivity
    • 11
    • 10
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5 pg/mL - 4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0382
    Supplier
    Rockland
    Purpose
    Human P-Cadherin Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human P-Cadherin. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 2 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human P-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human P-Cadherin standard solution and each sample be measured in duplicate.
    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: D108-G654

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all P-Cadherin (CDH3) ELISA Kits
    P-Cadherin (CDH3)
    Alternative Name
    P-Cadherin (CDH3 Products)
    Background

    Synonyms: AI385538, CADH3_HUMAN, Cadherin 3, Cadherin 3 precursor, Cadherin 3 type 1, Cadherin-3, Cadp, Calcium dependent adhesion protein placental, CDH 3, CDH3, CDH3 protein, CDHP, HJMD, P cadherin (placental), P-cadherin, PCAD, Placental cadherin

    Background: Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma. The standard product used in this kit is recombinant P-Cadherin with the molecular mass of 120-130Kda after glycosylation.

    Gene ID
    1001
    NCBI Accession
    NP_001304124
    UniProt
    P22223
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