IL7R ELISA Kit

Catalog No. ABIN6719851

Product Details IL7R ELISA Kit

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CD 127, CD127, CD127 antigen, CDw127, IL 7R alpha, IL 7R, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, IL7R, IL7RA, IL7RA_HUMAN, IL7Ralpha, ILRA, Interleukin 7 receptor alpha chain, Interleukin 7 receptor, Interleukin 7 receptor isoform H5 6, Interleukin-7 receptor subunit alpha

Background: The interleukin-7 receptor(IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7(IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain(IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V(D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.

Gene Name: IL7R

Production: Natural and recombinant human IL7R. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: E21-I262

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human IL7R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human IL7R standard solution and each sample be measured in duplicate.

ELISA Dilution: 312pg/mL-20000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: IL7R - CD127 (IL7R Products)

Synonyms: IL7R ELISA Kit, CD127 ELISA Kit, IL-7Ralpha ELISA Kit, CDW127 ELISA Kit, IL-7R-alpha ELISA Kit, IL7RA ELISA Kit, ILRA ELISA Kit, IL-7RA ELISA Kit, interleukin 7 receptor ELISA Kit, IL7R ELISA Kit, Il7r ELISA Kit

Gene ID: 3575

NCBI Accession: NP_002176

UniProt: P16871

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size