Neurotrophin 4 ELISA Kit

Catalog No. ABIN6719849

Product Details Neurotrophin 4 ELISA Kit

Target: Neurotrophin 4 (NTF4)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: GLC10, GLC1O, Neurotrophic factor 4, Neurotrophic factor 5, Neurotrophin 5, Neurotrophin 5 (neurotrophin 4/5), Neurotrophin-4, Neurotrophin-5, Neutrophic factor 4, Neutrophic factor 5, NT 4, NT 4/5, NT 5, NT-4, NT-5,NT4/5, NT4P, NT5, NTF4, NTF4_HUMAN, NTF5

Background: Neurotrophin-4(NT-4), also known as neurotrophin-5(NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons(2-4). NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.

Gene Name: NTF4

Production: Natural and recombinant human NT-4. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: sf21, Immunogen sequence: G81-A210

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NT-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human NT-4 standard solution and each sample be measured in duplicate.

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Neurotrophin 4

Target: Neurotrophin 4 (NTF4)

Alternative Name: NT-4 (NTF4 Products)

Synonyms: NTF5 ELISA Kit, GLC10 ELISA Kit, GLC1O ELISA Kit, NT-4 ELISA Kit, NT-4/5 ELISA Kit, NT-5 ELISA Kit, NT4 ELISA Kit, NT5 ELISA Kit, NT4P ELISA Kit, Ntf5 ELISA Kit, 2900040K06Rik ELISA Kit, AI462899 ELISA Kit, NT4/5 ELISA Kit, Ntf-5 ELISA Kit, Ntf4 ELISA Kit, neurotrophin 4 ELISA Kit, neurotrophin 5 ELISA Kit, NTF4 ELISA Kit, Ntf4 ELISA Kit, ntf4 ELISA Kit, Ntf5 ELISA Kit

Gene ID: 4909

NCBI Accession: NP_006170

UniProt: P34130

Pathways: RTK Signaling