CD47 ELISA Kit

Catalog No. ABIN6719833

Supplier Product No.: koa0858

Product Details CD47 ELISA Kit

Target: CD47

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125 pg/mL - 8000 pg/mL

Minimum Detection Limit: 125 pg/mL

Application: ELISA

Supplier Product No.: koa0858

Supplier: Rockland

Purpose: Human CD47 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CD47. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human CD47 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human CD47 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: Q19-P139

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CD47

Target: CD47

Alternative Name: CD47 (CD47 Products)

Background:

Synonyms: Antigen identified by monoclonal antibody 1D8, Antigenic surface determinant protein OA3, CD 47, CD47, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 antigen, CD47 glycoprotein, CD47 Molecule, CD47_HUMAN, IAP, Integrin Associated Protein, Integrin associated signal transducer, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, OA 3, OA3, OTTHUMP00000041152, OTTHUMP00000041153, Protein MER6, Rh related antigen

Background: CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.

Gene ID: 961

NCBI Accession: NP_001768

UniProt: Q08722