CD27 ELISA Kit

Catalog No. ABIN6719832

Product Details CD27 ELISA Kit

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CD 27, Cd27, CD27 antigen, CD27 Molecule, CD27_HUMAN, CD27L receptor, LPFS2, MGC20393, OTTHUMP00000238557, S152, T cell activation antigen CD27, T cell antivation antigen S152, T-cell activation antigen CD27, T14, TNFRSF 7, TNFRSF7, TNFSF7, Tp 55, Tp55, Tumor necrosis factor receptor superfamily member 7

Background: CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein(SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Gene Name: CD27

Production: Natural and recombinant mouse . There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: T21-R182

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse TNFRSF7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates and serum to each empty well. It is recommended that each mouse TNFRSF7 standard solution and each sample be measured in duplicate.

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: TNFRSF7 - CD27 (CD27 Products)

Synonyms: TNFRSF7 ELISA Kit, S152 ELISA Kit, Tnfrsf7 ELISA Kit, Tp55 ELISA Kit, S152. LPFS2 ELISA Kit, T14 ELISA Kit, CD27 molecule ELISA Kit, CD27 antigen ELISA Kit, CD27 ELISA Kit, Cd27 ELISA Kit

Gene ID: 21940

NCBI Accession: NP_001028298

UniProt: P41272

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints