Neurotrophin 3 ELISA Kit

Catalog No. ABIN6719810

Supplier Product No.: koa0279

Product Details Neurotrophin 3 ELISA Kit

Target: Neurotrophin 3 (NTF3)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Supplier Product No.: koa0279

Supplier: Rockland

Purpose: Human Neurotrophin-3 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human Neurotrophin-3. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Neurotrophin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants or serum to each empty well. It is recommended that each human Neurotrophin-3 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: sf21, Immunogen sequence: Y139-T257

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Alternative Name: Neurotrophin-3 (NTF3 Products)

Background:

Synonyms: AI316846, AI835689, HDNF, MGC129711, Nerve growth factor 2, Neurotrophic factor, Neurotrophin 3 (HDNF/NT 3), Neurotrophin 3 precursor, Neurotrophin3, NGF 2, NGF2, NT3, NTF 3, NTF3

Background: Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3, also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1

Gene ID: 4908

NCBI Accession: NP_001096124

UniProt: P20783

Pathways: RTK Signaling, Neurotrophin Signaling Pathway