Relaxin 1 ELISA Kit

Catalog No. ABIN6719784

Supplier Product No.: koa0723

Product Details Relaxin 1 ELISA Kit

Target: Relaxin 1 (RLN1)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78 pg/mL

Application: ELISA

Supplier Product No.: koa0723

Supplier: Rockland

Purpose: Mouse Relaxin 1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse Relaxin 1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse Relaxin 1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each mouse Relaxin 1 standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: R23-R54(B)&E161-C185(A)

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Relaxin 1

Target: Relaxin 1 (RLN1)

Alternative Name: Relaxin 1 (RLN1 Products)

Background:

Synonyms: H1RLX, Preprorelaxin H1, PreprorelaxinH1, Prorelaxin, Prorelaxin H1, ProrelaxinH1, REL1_HUMAN, Relaxin 1 (H1), Relaxin A chain, Relaxin H1, Relaxin1, RelaxinH1, RLN 1, RLN1, RLX H1, RLXH 1, RLXH

Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix(cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

Gene ID: 19773

NCBI Accession: NP_035402

UniProt: P47932