Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Prostate Specific Antigen ELISA Kit

Rockland koa0486 PSA Reactivity: Human Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Catalog No. ABIN6719774
Supplier Product No.: koa0486
  • Target See all Prostate Specific Antigen (PSA) ELISA Kits
    Prostate Specific Antigen (PSA)
    Reactivity
    • 15
    • 8
    • 7
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    312 pg/mL - 20000 pg/mL
    Minimum Detection Limit
    312 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0486
    Supplier
    Rockland
    Purpose
    Human Kallikrein 3 Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human Kallikrein 3. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 10 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product PSA ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Kallikrein 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Kallikrein 3 standard solution and each sample be measured in duplicate.
    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: A18-P261

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all Prostate Specific Antigen (PSA) ELISA Kits
    Prostate Specific Antigen (PSA)
    Alternative Name
    Kallikrein 3 (PSA Products)
    Background

    Synonyms: APS, Gamma seminoprotein, Gamma-seminoprotein, hK3, Kallikrein 3, Kallikrein related peptidase 3, Kallikrein-3, KLK 3, KLK2A1, KLK3, Klk3, KLK3_HUMAN, P 30 antigen, P-30 antigen, P30 antigen, Prostate Specific Antigen, Prostate-specific antigen, Psa, Semenogelase, Seminin

    Background: Prostate-specific antigen(PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland, PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome(19q13).3

    Gene ID
    354
    NCBI Accession
    NP_001025218
    UniProt
    P07288
    Pathways
    Complement System
You are here:
Support