NrCAM ELISA Kit

Catalog No. ABIN6719766

Product Details NrCAM ELISA Kit

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Bravo, hBravo, Neuronal cell adhesion molecule, Neuronal surface protein Bravo, Ng CAM related, Ng-CAM-related, NgCAM related cell adhesion molecule, NgCAM-related cell adhesion molecule, Nr CAM, Nr-CAM, Nrcam, NRCAM_HUMAN

Background: Neuronal cell adhesion molecule is a protein that in humans is encoded by the NRCAM gene. Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. Also, this gene is expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Name: NRCAM

Production: Natural and recombinant human NRCAM. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: L30-N600

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human NRCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human NRCAM standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for NrCAM

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Alternative Name: NRCAM (NRCAM Products)

Synonyms: CD56 ELISA Kit, MSK39 ELISA Kit, NCAM ELISA Kit, Bravo ELISA Kit, C030017F07Rik ELISA Kit, C130076O07Rik ELISA Kit, mKIAA0343 ELISA Kit, NRCAM ELISA Kit, si:dkey-240a12.1 ELISA Kit, Nr-CAM ELISA Kit, neural cell adhesion molecule 1 ELISA Kit, neuronal cell adhesion molecule ELISA Kit, neuronal cell adhesion molecule L homeolog ELISA Kit, neuronal cell adhesion molecule a ELISA Kit, NCAM1 ELISA Kit, NRCAM ELISA Kit, Nrcam ELISA Kit, nrcam.L ELISA Kit, nrcama ELISA Kit, nrcam ELISA Kit

Gene ID: 4897

NCBI Accession: NP_001032209

UniProt: Q92823

Pathways: Regulation of Cell Size