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alpha 2 Macroglobulin ELISA Kit

Rockland koa0638 A2M Reactivity: Human Colorimetric Sandwich ELISA 625 pg/mL - 40000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Catalog No. ABIN6719761
Supplier Product No.: koa0638
  • Target See all alpha 2 Macroglobulin (A2M) ELISA Kits
    alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
    Reactivity
    • 19
    • 10
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    625 pg/mL - 40000 pg/mL
    Minimum Detection Limit
    625 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0638
    Supplier
    Rockland
    Purpose
    Human A2M-alpha2-Macroglobulin Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural human A2M. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 20 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product A2M ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human A2M standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human A2M standard solution and each sample be measured in duplicate.
    Comment

    Standard: from human plasma

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all alpha 2 Macroglobulin (A2M) ELISA Kits
    alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
    Alternative Name
    A2M/alpha2-Macroglobulin (A2M Products)
    Background

    Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, A2m, A2MD, A2MG_HUMAN, Alpha-2-M, Alpha-2-macroglobulin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, CPAMD5, FWP007, Pregnancy zone protein, S863-7

    Background: Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease(AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates(activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

    Gene ID
    2
    NCBI Accession
    NP_000005
    UniProt
    P01023
    Pathways
    Lipid Metabolism
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