VEGF ELISA Kit
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- Target See all VEGF ELISA Kits
- VEGF (Vascular Endothelial Growth Factor (VEGF))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 31.2 pg/mL - 2000 pg/mL
- Minimum Detection Limit
- 31.2 pg/mL
- Application
- ELISA
- Supplier Product No.
- koa0329
- Supplier
- Rockland
- Purpose
- Human VEGF Sandwich ELISA Kit for Quantitative Detection
- Brand
- AccuSignal™
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
- Analytical Method
- Quantitative
- Specificity
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Production: Natural and recombinant human VEGF. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 1 pg/mL
- Components
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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- Application Notes
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each human VEGF standard solution and each sample is measured in duplicate.
- Comment
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Standard: Expression system for standard: sf21, Immunogen sequence: A27-R191
Detection Range: 31.2 pg/ml - 2000 pg/ml (serum, plasma), 15.6 pg/ml - 1000 pg/ml (cell culture supernates) - Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target See all VEGF ELISA Kits
- VEGF (Vascular Endothelial Growth Factor (VEGF))
- Alternative Name
- VEGF (VEGF Products)
- Background
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Synonyms: MGC70609, MVCD1, Vascular endothelial growth factor, Vascular endothelial growth factor A, Vascular permeability factor, VEGF A, VEGFA, VPF
Background: Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.
- Gene ID
- 7422
- NCBI Accession
- NP_001020537
- UniProt
- P15692
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