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FGF7 ELISA Kit

Rockland koa0490 FGF7 Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Rockland
Catalog No. ABIN6719748
Supplier Product No.: koa0490
  • Target See all FGF7 ELISA Kits
    FGF7 (Fibroblast Growth Factor 7 (FGF7))
    Reactivity
    • 9
    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2 pg/mL - 2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0490
    Supplier
    Rockland
    Purpose
    Human FGF7 - KGF Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human FGF7. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 4 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product FGF7 ELISA Kit
  • Application Notes

    ELISA_Dilution: 31.2pg/mL-2000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human FGF7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human FGF7 standard solution and each sample be measured in duplicate.

    Comment

    Standard: Expression system for standard: E.coli, Immunogen sequence: C32-T194

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all FGF7 ELISA Kits
    FGF7 (Fibroblast Growth Factor 7 (FGF7))
    Alternative Name
    FGF7/KGF (FGF7 Products)
    Background

    Synonyms: FGF 7, Fibroblast growth factor 7, HBGF 7, Heparin binding growth factor 7, Keratinocyte growth factor, KGF, OTTMUSP00000016341, OTTMUSP00000016342, RP23-386I21.3

    Background: Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.

    Gene ID
    2252
    NCBI Accession
    NP_002000
    UniProt
    P21781
    Pathways
    RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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