FGF7 ELISA Kit
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- Target See all FGF7 ELISA Kits
- FGF7 (Fibroblast Growth Factor 7 (FGF7))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 31.2 pg/mL - 2000 pg/mL
- Minimum Detection Limit
- 31.2 pg/mL
- Application
- ELISA
- Supplier Product No.
- koa0490
- Supplier
- Rockland
- Purpose
- Human FGF7 - KGF Sandwich ELISA Kit for Quantitative Detection
- Brand
- AccuSignal™
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
- Analytical Method
- Quantitative
- Specificity
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Production: Natural and recombinant human FGF7. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 4 pg/mL
- Components
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product FGF7 ELISA Kit
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- Application Notes
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ELISA_Dilution: 31.2pg/mL-2000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human FGF7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human FGF7 standard solution and each sample be measured in duplicate.
- Comment
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Standard: Expression system for standard: E.coli, Immunogen sequence: C32-T194
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target See all FGF7 ELISA Kits
- FGF7 (Fibroblast Growth Factor 7 (FGF7))
- Alternative Name
- FGF7/KGF (FGF7 Products)
- Background
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Synonyms: FGF 7, Fibroblast growth factor 7, HBGF 7, Heparin binding growth factor 7, Keratinocyte growth factor, KGF, OTTMUSP00000016341, OTTMUSP00000016342, RP23-386I21.3
Background: Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
- Gene ID
- 2252
- NCBI Accession
- NP_002000
- UniProt
- P21781
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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