CD5 ELISA Kit

Catalog No. ABIN6719736

Product Details CD5 ELISA Kit

Target: CD5

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 93.7 pg/mL - 6000 pg/mL

Minimum Detection Limit: 93.7 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CD 5, CD5, CD5 antigen (p56 62), CD5 antigen, CD5 Molecule, CD5_HUMAN, LEU 1, LEU1, Ly12, LyA, Lymphocyte Antigen CD5, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, OTTHUMP00000236973, p56 62, T cell surface glycoprotein CD5, T-cell surface glycoprotein CD5, T1

Background: CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli(such as bacterial proteins) and not by normal tissue proteins.

Gene Name: CD5

Production: Natural and recombinant human CD5. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: R25-N371

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/m human CD5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD5 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 93.7pg/mL-6000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CD5

Target: CD5

Alternative Name: soluble CD5 (CD5 Products)

Synonyms: CD5 ELISA Kit, Ly-1 ELISA Kit, Ly-12 ELISA Kit, Ly-A ELISA Kit, Lyt-1 ELISA Kit, LEU1 ELISA Kit, T1 ELISA Kit, CD5 molecule ELISA Kit, CD5 antigen ELISA Kit, CD5 molecule like ELISA Kit, Cd5 molecule ELISA Kit, CD5 ELISA Kit, Cd5 ELISA Kit, CD5L ELISA Kit

Gene ID: 921

NCBI Accession: NP_055022

UniProt: P06127