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CCL9 ELISA Kit

Ccl9 Reactivity: Mouse Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Catalog No. ABIN6719734
  • Target See all CCL9 (Ccl9) ELISA Kits
    CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))
    Reactivity
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    7.8 pg/mL - 500 pg/mL
    Minimum Detection Limit
    7.8 pg/mL
    Application
    ELISA
    Purpose
    Sandwich ELISA for Quantitative Detection of Antigen
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Characteristics

    Synonyms: CCF18, CCL9, Chemokine CC motif ligand 9, Macrophage Inflammatory Protein 1 gamma, Macrophage inflammatory protein-related protein 2, MIP-1 gamma, mMIP1g, SCYA10, SCYA9, Small inducible cytokine A9

    Background: Chemokine (C-C motif) ligand 9(CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium(FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1(the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.

    Gene Name: Ccl9

    Production: Natural and recombinant mouse CCL9 . There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: Q22-Q122

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  • Application Notes

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CCL9 standard solution and each sample is measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 7.8pg/mL-500pg/mL

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target See all CCL9 (Ccl9) ELISA Kits
    CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))
    Alternative Name
    CCL9 (Ccl9 Products)
    Synonyms
    CCF18 ELISA Kit, MRP-2 ELISA Kit, Scya10 ELISA Kit, Scya9 ELISA Kit, chemokine (C-C motif) ligand 9 ELISA Kit, Ccl9 ELISA Kit
    Gene ID
    20308
    NCBI Accession
    NP_035468
    UniProt
    P51670
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