CCL20 ELISA Kit

Catalog No. ABIN6719733

Product Details CCL20 ELISA Kit

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Beta chemokine exodus 1, Beta-chemokine exodus-1, C C motif chemokine 20, CC chemokine LARC, Ccl20, CCL20(2-70), CCL20_HUMAN, Chemokine (C C motif) ligand 20, CKb4, Exodus 1, LARC, Liver and activation regulated chemokine, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, MIP 3 alpha, MIP 3a, MIP-3-alpha, MIP3 alpha, MIP3A, MIP3alpha, SCYA20, Small inducible cytokine A20, Small inducible cytokine subfamily A (Cys Cys) member 20, Small-inducible cytokine A20, ST38

Background: Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

Gene Name: CCL20

Production: Natural and recombinant human MIP-3 alpha. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence:  A27-M96

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MIP-3 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human MIP-3 alpha standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: MIP-3 alpha - CCL20 (CCL20 Products)

Synonyms: MIP-3ALPHA ELISA Kit, CCL20 ELISA Kit, si:dkey-150o13.1 ELISA Kit, CKb4 ELISA Kit, LARC ELISA Kit, MIP-3-alpha ELISA Kit, MIP-3a ELISA Kit, MIP3A ELISA Kit, SCYA20 ELISA Kit, ST38 ELISA Kit, MIP-3A ELISA Kit, MIP-3[a] ELISA Kit, Scya20 ELISA Kit, exodus-1 ELISA Kit, C-C motif chemokine ligand 20 ELISA Kit, chemokine (C-C motif) ligand 20b ELISA Kit, chemokine (C-C motif) ligand 20 ELISA Kit, CCL20 ELISA Kit, ccl20b ELISA Kit, Ccl20 ELISA Kit

Gene ID: 6364

NCBI Accession: NP_001123518

UniProt: P78556

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection