S100A12 ELISA Kit

Catalog No. ABIN6719725

Product Details S100A12 ELISA Kit

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CAAF1, CAGC, Calcitermin, Calcium binding protein in amniotic fluid, Calcium-binding protein in amniotic fluid 1, Calgranulin C, Calgranulin related protein, Calgranulin-C, CGRP, EN RAGE, EN-RAGE, ENRAGE, Extracellular newly identified RAGE-binding protein, migration inhibitory factor-related protein 6, MRP6, Neutrophil S100 protein, p6, Protein S100 A12, S100 calcium binding protein A12, S100 calcium-binding protein A12 (calgranulin C), S100 calcium-binding protein A12, S10AC_HUMAN

Background: S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene. It is also known as calgranulin C. By direct R-banding FISH, the S100A12 gene is mapped to chromosome 1q21.2-q22. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. And this protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.

Gene Name: S100A12

Production: Natural and recombinant human S100A12. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: M1-E92

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human S100A12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human S100A12 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 312pg/mL-20,000pg/mL

Other Performance Data: DOE, 2-8° C - 04/19/19 -20° C - 10/18/19

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for S100A12

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Alternative Name: S100A12 (S100A12 Products)

Synonyms: CAAF1 ELISA Kit, CAGC ELISA Kit, CGRP ELISA Kit, ENRAGE ELISA Kit, MRP-6 ELISA Kit, MRP6 ELISA Kit, p6 ELISA Kit, CO-AG ELISA Kit, EN-RAGE ELISA Kit, S100 calcium binding protein A12 ELISA Kit, protein S100-A12 ELISA Kit, S100A12 ELISA Kit, LOC101103771 ELISA Kit

Gene ID: 6283

NCBI Accession: NP_005612

UniProt: P80511

Pathways: Toll-Like Receptors Cascades, S100 Proteins