ANGPTL3 ELISA Kit

Catalog No. ABIN6719722

Product Details ANGPTL3 ELISA Kit

Target: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: ANG 5, ANG-5, ANG5, Angiopoietin 5, Angiopoietin like 3, Angiopoietin related protein 3, Angiopoietin-5, Angiopoietin-like protein 3, Angiopoietin-related protein 3, ANGL3_HUMAN, ANGPT5, ANGPTL3, ANL3, FHBL2, OTTHUMP00000010719, UNQ153/PRO179

Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

Gene Name: ANGPTL3

Production: Natural and recombinant human ANGPTL3. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: sf21, Immunogen sequence: S17-E460

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ANGPTL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ANGPTL3 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 312pg/mL-20,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for ANGPTL3

Target: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Alternative Name: ANGPTL3 (ANGPTL3 Products)

Synonyms: fb60e11 ELISA Kit, fb66h02 ELISA Kit, wu:fb60e11 ELISA Kit, wu:fb66h02 ELISA Kit, zgc:111943 ELISA Kit, ANGPTL3 ELISA Kit, LOC100230785 ELISA Kit, ANG-5 ELISA Kit, ANGPT5 ELISA Kit, ANL3 ELISA Kit, FHBL2 ELISA Kit, hypl ELISA Kit, angiopoietin-like 3 ELISA Kit, angiopoietin like 3 ELISA Kit, angptl3 ELISA Kit, ANGPTL3 ELISA Kit, Angptl3 ELISA Kit

Gene ID: 27329

NCBI Accession: NP_055310

UniProt: Q9Y5C1