WISP1 ELISA Kit
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- Target See all WISP1 ELISA Kits
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 31.2 pg/mL - 2000 pg/mL
- Minimum Detection Limit
- 31.2 pg/mL
- Application
- ELISA
- Purpose
- Sandwich ELISA for Quantitative Detection of Antigen
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Characteristics
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Synonyms: CCN family member 4, CCN4, WISP1, WISP1c, WISP1i, WISP1tc, Wnt 1 inducible signaling pathway protein 1, Wnt-1 induced secreted protein, WNT1 induced secreted protein 1, WNT1 inducible signaling pathway protein 1, WNT1 inducible-signaling pathway protein 1
Background: WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84 % of tumors examined compared with patient-matched normal mucosa.
Gene Name: WISP1
Production: Natural and recombinant mouse WISP1. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: T23-N367
- Top Product
- Discover our top product WISP1 ELISA Kit
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- Application Notes
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse WISP1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse WISP1 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 31.2pg/mL-2000pg/mL
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Target See all WISP1 ELISA Kits
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
- Alternative Name
- WISP1 - CCN4 (WISP1 Products)
- Synonyms
- CCN4 ELISA Kit, WISP1c ELISA Kit, WISP1i ELISA Kit, WISP1tc ELISA Kit, WISP-1 ELISA Kit, WISP1 ELISA Kit, si:ch211-38f10.2 ELISA Kit, AW146261 ELISA Kit, Elm1 ELISA Kit, zgc:172276 ELISA Kit, WNT1 inducible signaling pathway protein 1 ELISA Kit, WNT1 inducible signaling pathway protein 1a ELISA Kit, WNT1-inducible-signaling pathway protein 1-like ELISA Kit, WNT1 inducible signaling pathway protein 1b ELISA Kit, WISP1 ELISA Kit, wisp1a ELISA Kit, Wisp1 ELISA Kit, LOC100627044 ELISA Kit, wisp1b ELISA Kit
- Gene ID
- 22402
- NCBI Accession
- NP_061353
- UniProt
- O54775
- Pathways
- WNT Signaling, Growth Factor Binding
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