PON1 ELISA Kit
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- Target See all PON1 ELISA Kits
- PON1 (Paraoxonase 1 (PON1))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 31.2 pg/mL - 2000 pg/mL
- Minimum Detection Limit
- 31.2 pg/mL
- Application
- ELISA
- Supplier Product No.
- koa0653
- Supplier
- Rockland
- Purpose
- Human PON1 Sandwich ELISA Kit for Quantitative Detection
- Brand
- AccuSignal™
- Sample Type
- Cell Culture Lysate, Cell Culture Supernatant, Plasma (heparin), Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
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Production: Natural and recombinant human PON1. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 10 pg/mL
- Components
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product PON1 ELISA Kit
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- Application Notes
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human PON1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin) or tissue homogenates to each empty well. It is recommended that each human PON1 standard solution and each sample be measured in duplicate.
- Comment
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Standard: Expression system for standard: NSO, Immunogen sequence: A2-L355
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target See all PON1 ELISA Kits
- PON1 (Paraoxonase 1 (PON1))
- Alternative Name
- PON1 (PON1 Products)
- Background
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Synonyms: A esterase 1, A-esterase 1, Aromatic esterase 1, Arylesterase 1, Arylesterase B type, ESA, Esterase A, K 45, K-45, MVCD5, Paraoxonase 1, Paraoxonase, Paraoxonase B type, Paraoxonase, plasma, Paraoxonase1, PON 1, PON, PON1, PON1_HUMAN, Serum aryldiakylphosphatase, Serum aryldialkylphosphatase 1, Serum paraoxonase/arylesterase 1
Background: Serum paraoxonase/arylesterase 1(PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1(paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein(HDL).
- Gene ID
- 5444
- NCBI Accession
- NP_000437
- UniProt
- P27169
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