SDC4 ELISA Kit

Catalog No. ABIN6719692

Supplier Product No.: koa0819

Product Details SDC4 ELISA Kit

Target: SDC4 (Syndecan 4 (SDC4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Supplier Product No.: koa0819

Supplier: Rockland

Purpose: Human Syndecan-4 - SDC4 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human Syndecan-4. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Syndecan-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human Syndecan-4 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: E19-E145

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for SDC4

Target: SDC4 (Syndecan 4 (SDC4))

Alternative Name: Syndecan-4/SDC4 (SDC4 Products)

Background:

Synonyms: Amphiglycan, MGC22217, OTTHUMP00000031788, ryudocan amphiglycan, Ryudocan, Ryudocan core protein, SDC 4, SDC4, SDC4_HUMAN, SYND 4, SYND4, syndecan 4, syndecan 4 (amphiglycan, ryudocan), syndecan proteoglycan 4, Syndecan-4, Syndecan4

Background: Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What's more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.

Gene ID: 6385

NCBI Accession: NP_002990

UniProt: P31431

Pathways: Glycosaminoglycan Metabolic Process