PINP ELISA Kit

Catalog No. ABIN6574243

Product Details PINP ELISA Kit

Target: PINP (Procollagen I N-Terminal Propeptide (PINP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78 pg/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PINP in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Procollagen I N-Terminal Propeptide (PINP)

Cross-Reactivity (Details): No significant cross-reactivity or interference between Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.

Sensitivity: 34 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Application Details

Comment:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Reagent Preparation:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 1.0mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 10,000pg/mL. Please firstly dilute the stock solution to 5,000pg/mL and the diluted standard serves as the highest standard (5,000pg/mL). Prepare 7 tubes containing 0.5mL Standard Diluent and produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL, and the last Tube with Standard Diluent is the blank as 0pg/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Sample Preparation:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage: 4 °C/-20 °C

Storage Comment:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Expiry Date: 6 months

References for PINP ELISA Kit (ABIN6574243)

Lei, Kilberg, Zemel, Rubenstein, Harris, Sheikh, Kelly, Kindler: "Bone metabolism and incretin hormones following glucose ingestion in young adults with pancreatic insufficient cystic fibrosis." in: Journal of clinical & translational endocrinology, Vol. 30, pp. 100304, (2022) (PubMed).

Abdul Alim, Domeij-Arverud, Nilsson, Edman, Ackermann: "Achilles tendon rupture healing is enhanced by intermittent pneumatic compression upregulating collagen type I synthesis." in: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, Vol. 26, Issue 7, pp. 2021-2029, (2018) (PubMed).

Sansoni, Vernillo, Perego, Barbuti, Merati, Schena, La Torre, Banfi, Lombardi: "Bone turnover response is linked to both acute and established metabolic changes in ultra-marathon runners." in: Endocrine, Vol. 56, Issue 1, pp. 196-204, (2016) (PubMed).

Rubiś, Wiśniowska-Śmiałek, Biernacka-Fijałkowska, Rudnicka-Sosin, Wypasek, Kozanecki, Dziewięcka, Faltyn, Karabinowska, Khachatryan, Hlawaty, Leśniak-Sobelga, Kostkiewicz, Płazak, Podolec: "Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR." in: Heart and vessels, Vol. 32, Issue 6, pp. 714-725, (2016) (PubMed).

Krege, Lane, Harris, Miller: "PINP as a biological response marker during teriparatide treatment for osteoporosis." in: Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, Vol. 25, Issue 9, pp. 2159-71, (2014) (PubMed).

Beranger, Pisani, Castel, Djedaini, Battaglia, Amiaud, Boukhechba, Ailhaud, Michiels, Heymann, Luquet, Amri: "Oxytocin reverses ovariectomy-induced osteopenia and body fat gain." in: Endocrinology, Vol. 155, Issue 4, pp. 1340-52, (2014) (PubMed).

Luthringer, Katha, Willumeit: "Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis." in: Journal of materials science. Materials in medicine, Vol. 25, Issue 11, pp. 2561-71, (2014) (PubMed).

Addison, Pond, Zhao, Mazzarello, Vandermeer, Goldstein, Amir, Clemons: "Effects of de-escalated bisphosphonate therapy on bone turnover biomarkers in breast cancer patients with bone metastases." in: SpringerPlus, Vol. 3, pp. 577, (2014) (PubMed).

Ji, Li, Xu, Wang, Luo, Luo, Ma, Li, Gong, He, Zhang, Yang, Zhou, Xiang, Wang: "IL4 and IL-17A provide a Th2/Th17-polarized inflammatory milieu in favor of TGF-?1 to induce bronchial epithelial-mesenchymal transition (EMT)." in: International journal of clinical and experimental pathology, Vol. 6, Issue 8, pp. 1481-92, (2013) (PubMed).

Koivula, Risteli, Risteli: "Measurement of aminoterminal propeptide of type I procollagen (PINP) in serum." in: Clinical biochemistry, Vol. 45, Issue 12, pp. 920-7, (2012) (PubMed).

Hryszko, Rydzewska-Rosolowska, Brzosko, Koc-Zorawska, Mysliwiec: "Low molecular weight iron dextran increases fibroblast growth factor-23 concentration, together with parathyroid hormone decrease in hemodialyzed patients." in: Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy, Vol. 16, Issue 2, pp. 146-51, (2012) (PubMed).

Eschalier, Jean, Pereira, Monzy, Vorilhon, Mactoux, Citron, Sapin, Motreff, Lusson: "Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial." in: Trials, Vol. 13, Issue 1, pp. 25, (2012) (PubMed).

Flynn: "Problems in strabismus management." in: Transactions of the New Orleans Academy of Ophthalmology, Vol. 34, pp. 449-60, (1986) (PubMed).

Target Details for PINP

Target: PINP (Procollagen I N-Terminal Propeptide (PINP))

Alternative Name: Procollagen I Propeptide (PINP) (PINP Products)

UniProt: P02452