CRP ELISA Kit

Catalog No. ABIN6574221

Product Details CRP ELISA Kit

Target: CRP (C-Reactive Protein (CRP))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CRP in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of C Reactive Protein (CRP)

Cross-Reactivity (Details): No significant cross-reactivity or interference between C Reactive Protein (CRP) and analogues was observed.

Sensitivity: 0.3 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Application Details

Comment:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Reagent Preparation:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 50 ng/mL. Prepare 7 tubes containing 0.5 mL Standard Diluent and produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 ng/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Sample Preparation:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage: 4 °C/-20 °C

Storage Comment:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

.

Expiry Date: 6 months

References for CRP ELISA Kit (ABIN6574221)

Stygar, Chełmecka, Sawczyn, Skrzep-Poloczek, Poloczek, Karcz: "Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague-Dawley Rats." in: Oxidative medicine and cellular longevity, Vol. 2018, pp. 2151429, (2018) (PubMed).

Olatunji, Olaniyi, Usman, Abolarinwa, Achile, Kim: "Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats." in: Environmental toxicology and pharmacology, Vol. 52, pp. 54-61, (2017) (PubMed).

Olatunji, Michael, Adeyanju, Areola, Soladoye: "Anti-inflammatory and antithrombotic effects of nicotine exposure in oral contraceptive-induced insulin resistance are glucocorticoid-independent." in: Pharmacological reports : PR, Vol. 69, Issue 3, pp. 512-519, (2017) (PubMed).

Zhang, Huang, Lu, Zhang, Cai: "Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs?" in: Clinical oral investigations, Vol. 20, Issue 7, pp. 1617-24, (2016) (PubMed).

Lavet, Martin, Linossier, Vanden Bossche, Laroche, Thomas, Gerbaix, Ammann, Fraissenon, Lafage-Proust, Courteix, Vico: "Fat and Sucrose Intake Induces Obesity-Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 31, Issue 1, pp. 98-115, (2016) (PubMed).

Olatunji, Usman, Seok, Kim: "Activation of cardiac renin-angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder." in: Archives of physiology and biochemistry, pp. 1-8, (2016) (PubMed).

Zhang, Fang, He, Guo, Runesson, Langel, Shi, Zhu, Bo: "Central Administration of Galanin Receptor 1 Agonist Boosted Insulin Sensitivity in Adipose Cells of Diabetic Rats." in: Journal of diabetes research, Vol. 2016, pp. 9095648, (2016) (PubMed).

Olatunji, Seok, Igunnu, Kang, Kim: "Combined oral contraceptive-induced hypertension is accompanied by endothelial dysfunction and upregulated intrarenal angiotensin II type 1 receptor gene expression." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 389, Issue 11, pp. 1147-1157, (2016) (PubMed).

Fu, Lei, Yin, Pan, Chai, Xu, Yan, Wang, Ke, Wu, Xu, Paranjpe, Qu, Nie: "Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis." in: Journal of ethnopharmacology, Vol. 195, pp. 118-126, (2016) (PubMed).

Chen, Zhang, Ge, Tseng, Bai, Qu, Beer, Autrup, Chen: "Subchronic toxicity and cardiovascular responses in spontaneously hypertensive rats after exposure to multiwalled carbon nanotubes by intratracheal instillation." in: Chemical research in toxicology, Vol. 28, Issue 3, pp. 440-50, (2015) (PubMed).

Bhattarai, Poudel, Kook, Lee: "Resveratrol prevents alveolar bone loss in an experimental rat model of periodontitis." in: Acta biomaterialia, Vol. 29, pp. 398-408, (2015) (PubMed).

Wang, Bose, Kim, Hong, Kim, Kim, Kim: "Flos Lonicera ameliorates obesity and associated endotoxemia in rats through modulation of gut permeability and intestinal microbiota." in: PLoS ONE, Vol. 9, Issue 1, pp. e86117, (2014) (PubMed).

Cakmak, Cavusoglu, Ates, Meral, Nacar, Erba?: "Regression of experimental endometriotic implants in a rat model with the angiotensin II receptor blocker losartan." in: The journal of obstetrics and gynaecology research, (2014) (PubMed).

Zhang, Huang, Qin, Ren: "Anti-inflammatory effect of Guan-Xin-Er-Hao via the nuclear factor-kappa B signaling pathway in rats with acute myocardial infarction." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 59, Issue 2, pp. 207-14, (2010) (PubMed).

Fetoni, Mancuso, Eramo, Ralli, Piacentini, Barone, Paludetti, Troiani: "In vivo protective effect of ferulic acid against noise-induced hearing loss in the guinea-pig." in: Neuroscience, Vol. 169, Issue 4, pp. 1575-88, (2010) (PubMed).

Li, Wang, Zeng, Feng, Cheng, Mao, Wang, Deng: "Metformin inhibits nuclear factor kappaB activation and decreases serum high-sensitivity C-reactive protein level in experimental atherogenesis of rabbits." in: Heart and vessels, Vol. 24, Issue 6, pp. 446-53, (2010) (PubMed).

Target Details for CRP

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: C Reactive Protein (CRP) (CRP Products)

Synonyms: PTX1 ELISA Kit, crp ELISA Kit, AI255847 ELISA Kit, Aa1249 ELISA Kit, Ab1-341 ELISA Kit, Ab2-196 ELISA Kit, Ac1-114 ELISA Kit, Ac1262 ELISA Kit, Ac2-069 ELISA Kit, Ba2-693 ELISA Kit, APCS ELISA Kit, 0610010I23Rik ELISA Kit, AW743261 ELISA Kit, C77570 ELISA Kit, CRP2 ELISA Kit, CRP4 ELISA Kit, Crp ELISA Kit, ESP1 ELISA Kit, Hlp ELISA Kit, CRP5.1 ELISA Kit, zgc:152809 ELISA Kit, C-reactive protein ELISA Kit, C-reactive protein, pentraxin-related ELISA Kit, c-reactive protein, pentraxin-related ELISA Kit, cysteine rich protein 2 ELISA Kit, CRP ELISA Kit, crp ELISA Kit, Crp ELISA Kit, Crip2 ELISA Kit, LOC776376 ELISA Kit

UniProt: P48199

Pathways: Carbohydrate Homeostasis