Src IQ-ELISA Kit

Catalog No. ABIN6386110

Product Details Src IQ-ELISA Kit

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity: Human

Detection Method: qPCR

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Immunoquantitative (PCR-Based) Src ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Semi-Quantitative

Characteristics: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Components:

• Src Microplate (Item A): 96 well PCR plate coated with anti-Human Src
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human Src
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for Src (Item F): 2 vials of a 4x concentrated solution of anti-Human Src affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Material not included:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Application Details

Application Notes: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Comment:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Sample Volume: 25 µL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: Src (Src Products)

Synonyms: AW259666 IQ-ELISA Kit, pp60c-src IQ-ELISA Kit, C-src1 IQ-ELISA Kit, CG7524 IQ-ELISA Kit, D-Src64B IQ-ELISA Kit, D-src IQ-ELISA Kit, DSRC64 IQ-ELISA Kit, DSrc IQ-ELISA Kit, DSrc64 IQ-ELISA Kit, DSrc64B IQ-ELISA Kit, Dm SRC1 IQ-ELISA Kit, DmHD-358 IQ-ELISA Kit, Dmel\CG7524 IQ-ELISA Kit, Dsrc IQ-ELISA Kit, Dsrc64 IQ-ELISA Kit, Dsrc64B IQ-ELISA Kit, HD-358 IQ-ELISA Kit, MRE5 IQ-ELISA Kit, SRC 64B IQ-ELISA Kit, Src IQ-ELISA Kit, Src1 IQ-ELISA Kit, Src64 IQ-ELISA Kit, c-src IQ-ELISA Kit, dSrc IQ-ELISA Kit, dsrc IQ-ELISA Kit, src IQ-ELISA Kit, src1 IQ-ELISA Kit, src64 IQ-ELISA Kit, src64B IQ-ELISA Kit, src64b IQ-ELISA Kit, ASV IQ-ELISA Kit, SRC1 IQ-ELISA Kit, c-SRC IQ-ELISA Kit, p60-Src IQ-ELISA Kit, c-Src IQ-ELISA Kit, PP60C-SCR IQ-ELISA Kit, SDR IQ-ELISA Kit, SRC proto-oncogene, non-receptor tyrosine kinase IQ-ELISA Kit, uncharacterized LOC5574802 IQ-ELISA Kit, proto-oncogene tyrosine-protein kinase src IQ-ELISA Kit, Rous sarcoma oncogene IQ-ELISA Kit, Src oncogene at 64B IQ-ELISA Kit, SRC IQ-ELISA Kit, LOC5574802 IQ-ELISA Kit, CpipJ_CPIJ010659 IQ-ELISA Kit, Src IQ-ELISA Kit, Src64B IQ-ELISA Kit

Target Type: Viral Protein

Gene ID: 6714

UniProt: P12931

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling