Reticulon 4 IQ-ELISA Kit

Catalog No. ABIN6385335

Product Details Reticulon 4 IQ-ELISA Kit

Target: Reticulon 4 (RTN4)

Reactivity: Human

Detection Method: qPCR

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Immunoquantitative (PCR-Based) Nogo-A (Reticulon 4A ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Semi-Quantitative

Sensitivity: 0.042 ng/mL

Characteristics: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Components:

• Nogo-A (Reticulon 4A Microplate (Item A): 96 well PCR plate coated with anti-Human Nogo-A (Reticulon 4A
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human Nogo-A (Reticulon 4A
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for Nogo-A (Reticulon 4A (Item F): 2 vials of a 4x concentrated solution of anti-Human Nogo-A (Reticulon 4A affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Material not included:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Application Details

Application Notes: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Comment:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Sample Volume: 25 µL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Reticulon 4 (RTN4)

Alternative Name: Nogo-A (Reticulon 4A) (RTN4 Products)

Synonyms: ASY IQ-ELISA Kit, NI220/250 IQ-ELISA Kit, NOGO IQ-ELISA Kit, NOGO-A IQ-ELISA Kit, NOGOC IQ-ELISA Kit, NSP IQ-ELISA Kit, NSP-CL IQ-ELISA Kit, Nbla00271 IQ-ELISA Kit, Nbla10545 IQ-ELISA Kit, Nogo-B IQ-ELISA Kit, Nogo-C IQ-ELISA Kit, RTN-X IQ-ELISA Kit, RTN4-A IQ-ELISA Kit, RTN4-B1 IQ-ELISA Kit, RTN4-B2 IQ-ELISA Kit, RTN4-C IQ-ELISA Kit, RTN4-Cw IQ-ELISA Kit, DKFZp459C0314 IQ-ELISA Kit, 1110020G17Rik IQ-ELISA Kit, AA407876 IQ-ELISA Kit, AA409940 IQ-ELISA Kit, AA960376 IQ-ELISA Kit, C130026I10Rik IQ-ELISA Kit, NgA IQ-ELISA Kit, Nogo-A IQ-ELISA Kit, mKIAA0886 IQ-ELISA Kit, mKIAA4153 IQ-ELISA Kit, NI-250 IQ-ELISA Kit, Nogo IQ-ELISA Kit, Vp20 IQ-ELISA Kit, r IQ-ELISA Kit, rat N IQ-ELISA Kit, rat NogoA IQ-ELISA Kit, reticulon-4 IQ-ELISA Kit, nogo IQ-ELISA Kit, rtn4 IQ-ELISA Kit, wu:fb17a02 IQ-ELISA Kit, wu:fb22f12 IQ-ELISA Kit, wu:fd61a07 IQ-ELISA Kit, zgc:92163 IQ-ELISA Kit, reticulon 4 IQ-ELISA Kit, reticulon 4a IQ-ELISA Kit, RTN4 IQ-ELISA Kit, rtn4 IQ-ELISA Kit, Rtn4 IQ-ELISA Kit, rtn4a IQ-ELISA Kit

Gene ID: 57142

UniProt: Q9NQC3

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome