Serotonin ELISA Kit

Catalog No. ABIN629513

Product Details Serotonin ELISA Kit

Target: Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Reactivity: Human

Method Type: Competition ELISA

Application: ELISA

Characteristics: ELISA kit for detection of Serotonin in the research laboratory

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Restrictions: For Research Use only

Handling

Precaution of Use: All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.

Storage: 4 °C

References for Serotonin ELISA Kit (ABIN629513)

Niziolek, Farmer, Cui, Turner, Warman, Robling: "High-bone-mass-producing mutations in the Wnt signaling pathway result in distinct skeletal phenotypes." in: Bone, Vol. 49, Issue 5, pp. 1010-9, (2011) (PubMed).

Target Details for Serotonin

Target: Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Alternative Name: Serotonin (5-HT Products)

Target Type: Chemical

Background: Serotonin is a monoamine neurotransmitter. Biochemically derived from tryptophan, serotonin is primarily found in the gastrointestinal (GI) tract, platelets, and in the central nervous system (CNS) of animals including humans. It is a well-known contributor to feelings of well-being, therefore it is also known as a happiness hormone despite not being a hormone.