Histamine ELISA Kit

Catalog No. ABIN629508

Product Details Histamine ELISA Kit

Target: Histamine (HIS)

Reactivity: Human

Method Type: Competition ELISA

Application: ELISA

Characteristics: ELISA kit for detection of Histamine in the research laboratory

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Restrictions: For Research Use only

Handling

Precaution of Use: All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.

Storage: 4 °C

Target Details for Histamine

Target: Histamine (HIS)

Alternative Name: Histamine (HIS Products)

Target Type: Chemical

Background: Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues.