(1 reference)-
- Target See all Testosterone ELISA Kits
- Testosterone
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Reactivity
- Hormone
- Method Type
- Competition ELISA
- Application
- ELISA
- Characteristics
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ELISA kit for detection of Testosterone in the research laboratory
- Top Product
- Discover our top product Testosterone ELISA Kit
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Testosterone ELISA Kit
Reactivity: Various Species Colorimetric Competition ELISA 49.4 pg/mL - 4000 pg/mL Plasma, Serum
Testosterone ELISA KitReactivity: Rat Colorimetric Competition ELISA 0.13-25.6 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Testosterone ELISA KitReactivity: Various Species Colorimetric Competition ELISA 0.31 ng/mL - 20 ng/mL Plasma, Saliva, Serum, Urine
Testosterone ELISA KitHigh Sensitivity Reactivity: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Testosterone ELISA KitSmall Sample Reactivity: Various Species Colorimetric Competition ELISA 370.4 pg/mL - 30000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Testosterone ELISA KitHigh Sensitivity Reactivity: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Testosterone ELISA KitReactivity: Pig Colorimetric Competition ELISA 0.1-20 ng/mL Plasma, Serum, Tissue Homogenate
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- Application Notes
- Optimal conditions to be determined by end user
- Assay Procedure
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The Testosterone ELISA Kit is a solid phase ELISA based on the principle of competitive binding. The microtiter wells are coated with an antibody directed towards an unique antigenic site on the Testosterone molecule. Endogenous Testosterone of a patient sample competes with a Testosterone horseradish peoxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is reverse proportional to the concentration of Testosterone in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of Testosterone in the patient sample.
- Restrictions
- For Research Use only
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- Precaution of Use
- All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.
- Storage
- 4 °C
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: "Timing of supplementation of selenium and isoflavones determines prostate cancer risk factor reduction in rats." in: Nutrition & metabolism, Vol. 5, pp. 31, (2009) (PubMed).
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: "Timing of supplementation of selenium and isoflavones determines prostate cancer risk factor reduction in rats." in: Nutrition & metabolism, Vol. 5, pp. 31, (2009) (PubMed).
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- Target See all Testosterone ELISA Kits
- Testosterone
- Abstract
- Testosterone Products
- Target Type
- Hormone
- Background
- Testosterone is a C19 steroid with an unsaturated bond between C-4 and C-5, a ketone group in C-3 a hydroxyl group in the Beta position at C-17 AND a molecular weight of 288.47. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes, in females ca. 50 % of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 20 % from the ovary and ca. 20 % from the adrenal glands.
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