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Progesterone ELISA Kit

Reactivity: Hormone Competition ELISA

Catalog No. ABIN629491

Datasheet as PDF

Target See all Progesterone ELISA Kits

Progesterone
Reactivity
8

4

4

3

3

3

3

2

2

2

1

1

1

1

1

1

1
Hormone

Method Type

Competition ELISA

Application

ELISA

Characteristics

ELISA kit for detection of Progesterone in the research laboratory

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Application Notes

Optimal conditions to be determined by end user

Assay Procedure

This Progesterone ELISA Kit is a ELISA based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site on the Progesterone molecule. Endogenous Progesterone of a patient sample competes with a Progesterone horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is reverse proportional to the concentration of Progesterone in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of Progesterone in the patient sample.

Restrictions

For Research Use only
Precaution of Use

All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.

Storage

4 °C
Target See all Progesterone ELISA Kits

Progesterone

Abstract

Progesterone Products

Target Type

Hormone

Background

Progesterone (pregn-4-ene-3, 20-dione) is a C21 steroid hormone containing a keto-group (at C-3) and a double bond between C-4 and C-5 ( Delta4). This steroid hormone is a female sex hormone which, in conjunction with estrogens, regulates the accessory organs during the menstrual cycle and it is particularly important in preparing the endometrium for the implantation of the blastocyte and in maintaining pregnancy.