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Thyroxine T4 ELISA Kit

T4 Reactivity: Hormone Colorimetric Competition ELISA

Catalog No. ABIN629485

Datasheet as PDF

Target See all Thyroxine T4 (T4) ELISA Kits

Thyroxine T4 (T4)
Reactivity
9

6

5

4

3

2

2

2

2

2

2

2

2

2

2

1
Hormone

Detection Method

Colorimetric

Method Type

Competition ELISA

Application

ELISA

Analytical Method

Quantitative

Characteristics

ELISA kit for detection of Thyroxine in the research laboratory
Alternative Names: Free T4 ELISA Kit

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Application Notes

Optimal conditions to be determined by end user

Plate

Pre-coated

Assay Procedure

The CBI fT4 is a solid phase competitive ELISA. The samples, assay buffer and T4 enzyme conjugate are added to the wells coated with anti-T4 monoclonal antibody. fT4 in the patient's serum competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound fT4 and T4 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of fT4 in the samples. A standard curve is prepared relating color intensity to the concentration of fT4.

Restrictions

For Research Use only
Precaution of Use

All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, and HbsAg. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.

Storage

4 °C
Target See all Thyroxine T4 (T4) ELISA Kits

Thyroxine T4 (T4)

Alternative Name

Thyroxine (T4 Products)

Target Type

Amino Acid

Background

The thyroid hormone, thyroxine (T4) is produced by the thyroid gland. An important component in the synthesis is iodine. The major form of thyroid hormone in the blood is thyroxine (T4). Thyroxine is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases (5'- iodinase). Thyroxine-binding globulin (TGB) is the major carrier protein for circulating thyroid hormone. Only a verysmall fraction of the circulating hormone is free (unbound) - T4 0.03 %.