Triiodothyronine T3 ELISA Kit

Catalog No. ABIN629484

Product Details Triiodothyronine T3 ELISA Kit

Target: Triiodothyronine T3 (T3)

Reactivity: Hormone

Method Type: Competition ELISA

Application: ELISA

Characteristics: ELISA kit for detection of Triiodothyronine in the research laboratory
Alternative Names: Free T3 ELISA Kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

The fT3 is a solid phase competitive ELISA. The samples, assay buffer and fT3 enzyme conjugate are added to the wells coated with anti-T3 monoclonal antibody. fT3 in the patient's serum competes with a T3 enzyme conjugate for binding sites. Unbound T3 and T3 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of fT3 in the samples. A standard curve is prepared relating color intensity to the concentration of the fT3.

Restrictions: For Research Use only

Handling

Precaution of Use: All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, and HbsAg. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.

Storage: 4 °C

Target Details for Triiodothyronine T3

Target: Triiodothyronine T3 (T3)

Alternative Name: Triiodothyronine (T3 Products)

Target Type: Amino Acid

Background: Triiodothyronine (T3), is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate. Production of T3 and its prohormone thyroxine (T4) is activated by thyroid-stimulating hormone (TSH), which is released from the pituitary gland. As the true hormone, the effects of T3 on target tissues are roughly four times more potent than those of T4.